Comparison of p16 with human papillomavirus with oral squamous cell carcinoma and oral premalignant lesion

Human Papillomavirus with Oral Squamous Cell Carcinoma


  • Mohammad Manzoor Pathology Department, Bacha Khan Medical College Mardan
  • Muhammad Zaib Khan Dental Surgeon Consultant, Endodontics, Safari Hospital, Rawalpindi
  • Adnan Sarwar Department of Pathology, Pak International Medical College, Peshawar
  • Fatima Daud Department of Anatomy, Pak International Medical College, Peshawar
  • Inam-u llah Department of Food Science, The University of Haripur, KPK, Pakistan
  • Hina Mir Department of Biochemistry, Shaheed Benazir Bhutto Women University, Peshawar
  • Bushra Tahreem Department of Life Sciences, School of Science, University of Management and Technology, Lahore Pakistan
  • Muhammad Awais Department of Life Sciences, School of Science, University of Management and Technology, Lahore Pakistan
  • Sudhair Abbas Bangash Faculty of Life Science, Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar
  • Fares M Muthanna Department of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand



P16; Oral Premalignant Lesions; Oral Squamous Cell Carcinoa; Human Papillomavirus (16/18)


Worldwide Oral carcinomas considerable problem. It is crucial to know the molecular incidences of molecular carcinogenesis, as it has a significant association with tobacco in Pakistani population. So, this study aimed to screen out the savage high risk of papilloma virus 16/18 and p16 in oral premalignant lesion and oral squamous cell carcinoma. Moreover, the p16 correlation between HPV and OPL and OSCC was also analyzed. Method: A total of 150 samples from the oral cavity were taken from the Hayatabad Medical complex (50 samples of OPL and 100 samples of OSCC).In this study we used immunohistochemistry to look for p16 in OPL and OSCC, and we used polymerase chain reaction to find HPV. SPSS 21 was used to input and evaluate the data. The relationship between HPVandp16withdifferentvariables was determined using Chi-square and Fisher exact tests. Results:For p16, 14% of 50 oral premalignant lesions were found positive and 86% were negative. Moreover, while screening for the OSCC, the 18 percent individuals were found positive for OSCC, while the remaining (82%) screened out negative. HPV was diagnosed in 6% of 50 OPL. The co-occurrence of HPV with p16 was found in all the 15 (100%) individual’s with a p value of 0.001. However, three of the 18 cases with p16 expression did not have HPV infection. Conclusion: The current research supports the use of p16 as a unique marker for human papilloma virus in oral squamous cell carcinomas. Furthermore, a chemical carcinogen like cigarettes is thought to be one of the main risk factors for p16 and HPV infection, as well as other things.


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DOI: 10.54393/pbmj.v4i2.230
Published: 2021-12-30

How to Cite

Manzoor, M. ., Khan, M. Z. ., Sarwar, A. ., Daud, F. ., llah, I.- u, Mir, H., Tahreem, B. ., Awais, M. ., Bangash, S. A., & Muthanna, F. M. . (2021). Comparison of p16 with human papillomavirus with oral squamous cell carcinoma and oral premalignant lesion: Human Papillomavirus with Oral Squamous Cell Carcinoma. Pakistan BioMedical Journal, 4(2), 287–291.



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