Clinical Correlation of Dengue Strains on the Basis of Seroprevalence in a Tertiary Care Hospital.

Clinical Correlation of Dengue Strains

Authors

  • Ali Haider University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
  • Faheem Ullah University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
  • Muhammad Bilal University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
  • Zeeshan Saif University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan
  • Hina Awais The University of lahore
  • Talha Mannan Department of Medical Lab technology, Shalamar School of Allied Health Sciences, Lahore, Pakistan
  • Hasnain Javed Provincial Public Health Reference Lab, Punjab AIDS Control Program, Lahore, Pakistan
  • Fareeha Bashir University Institute of Medical Lab Technology, The University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i4.341

Keywords:

Dengue, Reverse transcriptase polymerase chain reaction, Enzyme linked immunosorbent assay, White blood cells and Platelets

Abstract

Dengue viruses are icosahedral in structure and contain a single-stranded positive- sense RNA sequence of 11kb inside their capsid protein, which belongs to Flaviviridae family, genera Flavivirus. DENV a vector dependent viral virus which presents a severe health danger worldwide. Objective: To study the different strains of dengue  on the basis of serotypes Methods: A cross sectional study was conducted at a tertiary care hospital Lahore, Primary and Secondary Health Department Lahore. The blood samples of 103 patients were collected from non-random sampling technique to check out the data of different parameters such as WBC’s & Platelets through Complete Blood Count (CBC), NS1 from ELISA and nature of dengue strains through RT- PCR. Results: Of 103 positive dengue patients, there were n= 58 (33.63 ± 16.54) males and n=45 (40.64± 16.00) females. Data for the total patients is subjected to statistical differences by Paired t-test (*p<0.05). The overall percentage of dengue strains within the sample population was DENV-2 (96%), DENV-3 (2%), DENV-1 & DENV-2 (2% in females, nil in males) DENV-2 & DENV-3 (2% both in males and females). According to clinical parameters the correlation of ELISA results with WBC’s was significant (<0.0001) as well as ELISA and platelets of patients had no correlation with each other. Conclusions: Leukopenia and Thrombocytopenia is found particularly in DENV-2 strain as well as we found two different strains in two patients. So, our research work is helpful for the identification in genetic similarity of dengue strains.

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Published

2022-04-30

How to Cite

Haider, A. ., Ullah, F. ., Bilal, M. ., Saif, Z., Awais, H., Mannan, T., Javed, H. ., & Bashir, F. . (2022). Clinical Correlation of Dengue Strains on the Basis of Seroprevalence in a Tertiary Care Hospital.: Clinical Correlation of Dengue Strains. Pakistan BioMedical Journal, 5(4), 149–153. https://doi.org/10.54393/pbmj.v5i4.341

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