Phenotypic Detection Of Antibiotic Resistance And Production Of Extended Spectrum Beta lactamases In E.COLI Isolated From UTI Patients At (HMC)

Phenotypic detection of antibiotic resistance and beta-lactamases in e. coli isolated from UTI

Authors

  • Umair AKbar Institute of Biotechnology & Microbiology Bacha Khan University Charsadda, Kp Pakistan
  • Muhammad Ali Institute of Biotechnology and Microbiology Bacha Khan University Charsadda, KPK Pakistan
  • Fawad Ali Institute of Biotechnology and Microbiology Bacha Khan University Charsadda, KPK Pakistan
  • Yamin Rashid SR Medicine & Allied, Swat Medical College, Swat, KPK, Pakistan
  • Ikramullah . Department of Agriculture, Bacha Khan University, Charsadda, KPK, Pakistan
  • Khan Niaz Khan Department of Biology, Edwardes College Peshawar, KPK, Pakistan

DOI:

https://doi.org/10.54393/pbmj.v5i4.360

Keywords:

antibiotic resistance, E. coli, beta-lactamases, UTI patients

Abstract

This study aimed at the isolation of antibiotic resistance and ESBL producing E. coli from urinary tract infection patients visiting HMC hospital Peshawar. Method: A total of 200 samples were collected and cultured on selective media for bacterial isolation. Two hundred isolates were subjected to different morphological and biochemical tests for the isolation and identification of E. coli. A total of 121 E. coli isolations were recovered. These E. coli isolates were subjected to antibiotic sensitivity testing. Eight antibiotics (Meropenem, Tazocin, Ciprofloxacin, Fosfomycin, Cefotaxime, Augmentin, Ceftazidime, Cefepime) were used for this purpose. Result: The isolates showed high resistance to these antibiotics such as meropenem having percent resistance of 1.85 while sensitivity is 98.14%, Tazocin showed percent resistance of 9.25 and sensitivity is 85.18%, Ciprofloxacin showed resistance of 87.96 percent, sensitivity is 12.03%, Fosfomycin showed resistance of 3.70 percent and sensitivity is 95.37%, Cefotaxime, Augmentin, Ceftazidime, Cefepime showed resistance of 100 percent. The isolates were then investigated for the existence of ESBL enzymes production through double disc diffusion test. Among the 121 isolates, 108 were positive for ESBL enzymes production. Conclusion: Current findings highlight the high prevalence of multi drug resistant and ESBL positive isolates of E. coli in clinical samples. Proper management and control measures are needed to control the spread of these highly pathogenic E. coli isolates to ensure public health safety.

References

Yang X, Wang H. Pathogenic E. coli (introduction), p 695-701. Encyclopedia of food microbiology, 2nd ed. Academic Press, Oxford, UK. 2014. doi.org/10.1016/B978-0-12-384730-0.00383-9

https://doi.org/10.1016/B978-0-12-384730-0.00383-9

Leclerc HD, Mossel DA, Edberg SC, Struijk CB. Advances in the bacteriology of the coliform group: their suitability as markers of microbial water safety. Annual Reviews in Microbiology. 2001,55(1): 201-34.doi.org/10.1146/annurev.micro.55.1.201

https://doi.org/10.1146/annurev.micro.55.1.201

Bavaro MF. E. coli O157: H7 and other toxigenic strains: the curse of global food distribution. Current gastroenterology reports. 2012,14(4):317-23. doi.org/10.1007/s11894-012-0264-6

https://doi.org/10.1007/s11894-012-0264-6

Castellani A, Chalmers AJ. Manual of tropical medicine. Baillière, Tindall and Cox; 1919.doi.org/10.5962/bhl.title.84653

https://doi.org/10.5962/bhl.title.84653

Croxen MA, Finlay BB. Molecular mechanisms of Escherichia coli pathogenicity. Nature Reviews Microbiology. 2010,8(1): 26-38.doi.org/10.1038/nrmicro2265

https://doi.org/10.1038/nrmicro2265

Brüssow H, Rahim H, Freire W. Epidemiological analysis of serologically determined rotavirus and enterotoxigenic Escherichia coli infections in Ecuadorian children. Journal of clinical microbiology. 1992,30(6):1585-7. doi.org/10.1128/jcm.30.6.1585-1587.1992

https://doi.org/10.1128/jcm.30.6.1585-1587.1992

Regua AH, Bravo VL, Leal MD, Lobo Leite ME. Epidemiological survey of the enteropathogenic Escherichia coli isolated from children with diarrhea. Journal of tropical pediatrics. 1990,36(4): 176-9.doi.org/10.1093/tropej/36.4.176

https://doi.org/10.1093/tropej/36.4.176

Khan IU, Mirza IA, Ikram A, Ali S, Hussain A, et al. In vitro activity of fosfomycin tromethamine against extended spectrum beta-lactamase producing urinary tract bacteria. J Coll Physicians Surg Pak. 2014,24(12):914-17.

Liu HY, Lin HC, Lin YC, Yu SH, Wu WH, et al. Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan. Journal of Microbiology, Immunology and Infection. 2011,44(5): 364-8.doi.org/10.1016/j.jmii.2010.08.012

https://doi.org/10.1016/j.jmii.2010.08.012

Shahzad K A, Ullah F, Muhammad K, Khatoon F, Hassan Qazi M, et al. "Multiple Drug Resistance Pattern in Urinary Tract Infection Short Communication ARTICLE HISTORY ABSTRACT". J Infect Mol Biol, 2013,4(1):67-70.

Gupta K, Hooton TM, Stamm WE. Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Annals of internal medicine. 2001,135(1): 41-50.doi.org/10.7326/0003-4819-135-1-200107030-00012

https://doi.org/10.7326/0003-4819-135-1-200107030-00012

Jafri SA, Qasim M, Masoud MS. Antibiotic resistance of E. coli isolates from urine samples of Urinary Tract Infection (UTI) patients in Pakistan. Bioinformation. 2014,10(7):419. doi.org/10.6026/97320630010419

https://doi.org/10.6026/97320630010419

Kibret M, Abera B. Antimicrobial susceptibility patterns of E. coli from clinical sources in northeast Ethiopia. African health sciences. 2011,11: 40-5.doi.org/10.4314/ahs.v11i3.70069

https://doi.org/10.4314/ahs.v11i3.70069

Scharff AZ, Rousseau M, Mariano LL, Canton T, Albert ML, Fontes M, Duffy D, Ingersoll MA. Sex differences in IL-17 determine chronicity in male versus female urinary tract infection. bioRxiv. 2018: 449124.doi.org/10.1101/449124

https://doi.org/10.1101/449124

Dielubanza EJ, Schaeffer AJ. Urinary tract infections in women. Medical clinics. 2011,95(1): 27-41.doi.org/10.1016/j.mcna.2010.08.023

https://doi.org/10.1016/j.mcna.2010.08.023

Linhares I, Raposo T, Rodrigues A, Almeida A. Frequency and antimicrobial resistance patterns of bacteria implicated in community urinary tract infections: a ten-year surveillance study (2000-2009). BMC infectious diseases. 2013,13(1): 1-4.doi.org/10.1186/1471-2334-13-19

https://doi.org/10.1186/1471-2334-13-19

van Driel AA, Notermans DW, Meima A, Mulder M, Donker GA, et al. Antibiotic resistance of Escherichia coli isolated from uncomplicated UTI in general practice patients over a 10-year period. European Journal of Clinical Microbiology & Infectious Diseases. 2019,38(11):2151-8. doi.org/10.1007/s10096-019-03655-3

https://doi.org/10.1007/s10096-019-03655-3

Kumar S, Dave A, Wolf B, Lerma EV. Urinary tract infections. Dis Mon. 2015,61:45-59. doi: 10.1016/j.disamonth.2014.12.002.

https://doi.org/10.1016/j.disamonth.2014.12.002

Turpin C, Minkah B, Danso K, Frimpong E. Asymptomatic Bacteriuria in Pregnant Women Attending Antenatal Clinic at Komfo Anokye Teaching Hospital, Kumasi, Ghana. Ghana Med J. 2007,41(1):26-29.

Kazemier BM, Schneeberger C, De Miranda E, Wassenaer AV, Bossuyt PM, et al. Costs and effects of screening and treating low risk women with a singleton pregnancy for asymptomatic bacteriuria, the ASB study. BMC Pregnancy Childbirth. 2012,12:52. doi:10.1186/1471-2393-12-

https://doi.org/10.1186/1471-2393-12-52

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Published

2022-04-30

How to Cite

AKbar, U. ., Ali , M. ., Ali, F., Rashid, Y. ., ., I., & Khan , K. N. (2022). Phenotypic Detection Of Antibiotic Resistance And Production Of Extended Spectrum Beta lactamases In E.COLI Isolated From UTI Patients At (HMC): Phenotypic detection of antibiotic resistance and beta-lactamases in e. coli isolated from UTI. Pakistan BioMedical Journal, 5(4), 103–108. https://doi.org/10.54393/pbmj.v5i4.360

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