Susceptibility Pattern of Pathogenic Bacteria “Pseudomonas aeruginosa” to various Antibiotics isolated from Post-Surgical Wound of Diabetic Patient at Hayatabad Medical Complex (HMC) Peshawar

Susceptibility Pattern of Pathogenic Bacteria “Pseudomonas aeruginosa”

Authors

  • Syed Saifur Shah Institute of Biotechnology & Microbiology Bacha Khan University Charsadda, Kp Pakistan
  • Muhammad Ali Institute of Biotechnology & Microbiology Bacha Khan University Charsadda, Kp Pakistan.
  • Fawad Ali Institute of Biotechnology & Microbiology Bacha Khan University Charsadda, Kp Pakistan
  • Yamin Rashid SR Medicine & Allied, Swat Medical college Swat, KPK, Pakistan
  • Ikramullah . Department of Agriculture Bacha Khan University Charsadda, Kp Pakistan
  • Khan Niaz Khan Department of Biology Edwardes College Peshawar, KPK, Pakistan
  • Farrah Shams Anatomy Department, Batterjee Medical College, Jeddah, Saudi Arabia

DOI:

https://doi.org/10.54393/pbmj.v5i4.355

Keywords:

P. aeruginosa, Diabetics patients, diffusion Method

Abstract

Pseudomonas aeruginosa is a gram-negative rod shape opportunistic bacteria involved in nosocomial infection and resistant to most of the commonly used antibiotics. Objective: To find out the antibiotic susceptibly pattern of P. aeruginosa. Methods: The current study was carried out on a sample of Diabetics patients having post-operative wounds in Hyatabad medical Complex. The pus sample was screened of pathogen bacteria P. aeruginosa was isolated from most of the sample i.e. 56% while other microorganisms isolated were i.e.  E coli, S. aureus, Proteus volugarus. Klebseilla spp coliform, Aclnelobacter spp, Proteus merabillus Provedentia spp. For the identification, different tests were performed including culture growth characteristic Gram staining, biochemical test. Antibiotics susceptibility test where performed utilizing disc diffusion method on Mueller Hinton ager. Results: Out of 200 samples, 112 (56%) sample were positive for P. aeruginosa. Infection rate was high in male than female (2:1). The date of the admitted patients in hospital show higher chance of infection as compare of out-patient department (OPD). Among the bacterial isolated 67% where resistant and 31 % were sensitive to cefotaxime, 38% resistance while 61% were sensitive to ceftazidime, 15% resistance and 80 % were sensitive to Meropenem. 10 % resistance to and 86% sensitive to lmipenem, 3.5% resistance and 96% sensitive to tazocin and 6.3 % resistant while 87% were sensitive to sulzon, using disc diffusion method. Conclusions: Meropenem, lmipenem, tazocin and sulzon were formed to be the most effective agents isolated P. aeruginosa isolates. The results of the bacterial isolates did not showed 100% susceptibility against any of the tested antibiotics. In this study, it is concluded that Tazocin and Sulzon is the choice of drug for the SSI of diabetic patients.

References

Ott E, Saathoff S, Graf K, Schwab F and Chaberny IF. The prevalence of nosocomial and community acquired infections in a university hospital: an observational study. Dtsch Arztebl Int. 2013;110(31-32):533-40. doi: 10.3238/arztebl.2013.0533.

https://doi.org/10.3238/arztebl.2013.0533

Health Quality Ontario. Portable ultraviolet light surface-disinfecting devices for prevention of hospital-acquired infections: a health technology assessment. Ontario health technology assessment series. 2018;18(1):1.

Zimmerli W and Sendi P. Pathogenesis of implant-associated infection: the role of the host. Semin Immunopathol. 2011;33(3):295-306. doi: 10.1007/s00281-011-0275-7.

https://doi.org/10.1007/s00281-011-0275-7

Erbay H, Yalcin AN, Serin S, Turgut H, Tomatir E and Cetin B et al. Nosocomial infections in intensive care unit in a Turkish university hospital: a 2-year survey. Intensive Care Med. 2003;29(9):1482-8. doi: 10.1007/s00134-003-1788-x.

https://doi.org/10.1007/s00134-003-1788-x

Zhang K, Sparling J, Chow BL, Elsayed S, Hussain Z and Church DL et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J Clin Microbiol. 2004;42(11):4947-55. doi: 10.1128/JCM.42.11.4947-4955.2004.

https://doi.org/10.1128/JCM.42.11.4947-4955.2004

Ziebuhr W. Staphylococcus aureus and Staphylococcus epidermidis: emerging pathogens in nosocomial infections. Contrib Microbiol. 2001;8:102-7. doi: 10.1159/000060402.

https://doi.org/10.1159/000060402

Bowler PG, Duerden BI and Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;14(2):244-69. doi: 10.1128/CMR.14.2.244-269.2001.

https://doi.org/10.1128/CMR.14.2.244-269.2001

Anthony A, Anthony I and Steve J. Studies on multiple antibiotic resistant bacterial isolated from surgical site infection. Scientific Research and Essays. 2010;5(24):3876-81. doi.org/10.5897/SRE.9000054.

Brodsky MH and Nixon MC. Rapid method for detection of Pseudomonas aeruginosa on MacConkey agar under ultraviolet light. Appl Microbiol. 1973;26(2):219-20. doi: 10.1128/am.26.2.219-220.1973.

https://doi.org/10.1128/am.26.2.219-220.1973

Olee A. Isolation and identification of pathogenic bacteria from college premises (Doctoral dissertation, St. Xavier's College). 2016.

Ruangpan L and Tendencia E. Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Aquaculture Department, Southeast Asian Fisheries Development Center. 2004:13-29.

Armstrong DG and Lipsky BA. Diabetic foot infections: stepwise medical and surgical management. Int Wound J. 2004;1(2):123-32. doi: 10.1111/j.1742-4801.2004.00035.x.

https://doi.org/10.1111/j.1742-4801.2004.00035.x

Islam MA, Alam MM, Choudhury ME, Kobayashi N and Ahmed MU. Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of methicillin-resistant Staphylococcus aureus (MRSA) with their antibiogram. Bangladesh Journal of Veterinary Medicine. 2008;6(1):121-126. doi.org/10.3329/bjvm.v6i1.1350.

https://doi.org/10.3329/bjvm.v6i1.1350

Zapata A and Ramirez-Arcos S. A comparative study of McFarland turbidity standards and the Densimat photometer to determine bacterial cell density. Curr Microbiol. 2015;70(6):907-9. doi: 10.1007/s00284-015-0801-2.

https://doi.org/10.1007/s00284-015-0801-2

Fugelsang KC, Edwards CG, editors. Wine microbiology: practical applications and procedures. Boston, MA: Springer US. 2007.

https://doi.org/10.1007/978-0-387-33349-6

Altmann G, Eisenberg E and Bogokowsky B. Radiation sterilization of triple sugar iron agar. The International Journal of Applied Radiation and Isotopes. 1979;30(9):527-529. doi.org/10.1016/0020-708X(79)90165-0.

https://doi.org/10.1016/0020-708X(79)90165-0

Kamtikar R and Mitra N. Clinico microbiological profile of pseudomonas aeruginosa isolated from diabetic foot ulcer. J. Sci. Innov. Res. 2014;3:478-81.

Lam DS, Houang E, Fan DS, Lyon D, Seal D and Wong E et al. Incidence and risk factors for microbial keratitis in Hong Kong: comparison with Europe and North America. Eye (Lond). 2002;16(5):608-18. doi: 10.1038/sj.eye.6700151.

https://doi.org/10.1038/sj.eye.6700151

Xu Z, Lin X, Soteyome T, Ye Y, Chen D and Yang L et al. Significant downtrend of antimicrobial resistance rate and rare β-lactamase genes and plasmid replicons carriage in clinical Pseudomonas aeruginosa in Southern China. Microbial Pathogenesis. 2021;159:105124. doi.org/10.1016/j.micpath.2021.105124.

https://doi.org/10.1016/j.micpath.2021.105124

Nasirmoghadas P, Yadegari S, Moghim S, Esfahani BN, Fazeli H and Poursina F et al. Evaluation of Biofilm Formation and Frequency of Multidrug-resistant and Extended Drug-resistant Strain in Pseudomonas aeruginosa Isolated from Burn Patients in Isfahan. Adv Biomed Res. 2018;7:61. doi: 10.4103/abr.abr_37_17.

https://doi.org/10.4103/abr.abr_37_17

Pope CF. Evolution of fluoroquinolone resistance in Burkholderia cepacia. University of London, University College London (United Kingdom). 2008.

Asmat U, Mumtaz MZ and Malik A. Rising prevalence of multidrug-resistant uropathogenic bacteria from urinary tract infections in pregnant women. J Taibah Univ Med Sci. 2020;16(1):102-111. doi: 10.1016/j.jtumed.2020.10.010.

https://doi.org/10.1016/j.jtumed.2020.10.010

Yang MM, Zhao HH, Ding XQ, Zhu GH, Yang ZH and Ding L et al. Self-Care Behavior of Hemodialysis Patients With Arteriovenous Fistula in China: A Multicenter, Cross-Sectional Study. Ther Apher Dial. 2019;23(2):167-172. doi: 10.1111/1744-9987.12770.

https://doi.org/10.1111/1744-9987.12770

Ahmed J, Jan AH, Nawaz G and Khan M. Epidemiology and antibiotic susceptibility of bacterial isolates from Northern Pakistan. African Journal of Microbiology Research. 2011;5(28):4949-55. doi.org/10.5897/AJMR10.368.

Yang X, Xing B, Liang C, Ye Z and Zhang Y. Prevalence and fluoroquinolone resistance of pseudomonas aeruginosa in a hospital of South China. Int J Clin Exp Med. 2015;8(1):1386-90.

Downloads

Published

2022-04-30

How to Cite

Shah, S. S., Ali, M. ., Ali, F., Rashid, Y. ., ., I., Khan, K. N. ., & Shams, F. . (2022). Susceptibility Pattern of Pathogenic Bacteria “Pseudomonas aeruginosa” to various Antibiotics isolated from Post-Surgical Wound of Diabetic Patient at Hayatabad Medical Complex (HMC) Peshawar: Susceptibility Pattern of Pathogenic Bacteria “Pseudomonas aeruginosa”. Pakistan BioMedical Journal, 5(4), 96–102. https://doi.org/10.54393/pbmj.v5i4.355

Issue

Section

Original Article

Most read articles by the same author(s)