Effectiveness of Dietary Supplementation in women with PCOS: A randomized-controlled trial

Effectiveness of Dietary Supplementation in women with PCOS


  • Hafsah Gul Khattak Ibadat International University Islamabad
  • Mazhar Ali Bhutto Physiotherapy Department, Govt Civil Hospital Khairpur
  • Muhammad Khan Bugti Human and Rehabilitation Department, Begum Nusrat Bhutto University, Sukkur
  • Huda Anjum Shifat Tameer-e-Millat University Islamabad
  • Maryum Fatima University of Lahore, Gujrat Campus
  • Hafiza Sabeen Ayesha Agile Institute of Rehabilitation Sciences Bahawalpur
  • Asma Ishtiaq Ibadat International University Islamabad
  • Aliza Farooq Ibadat International University Islamabad
  • Maliva Latif Ibadat International University Islamabad




PCOS, Vitamin D, Plasma glucose, Insulin


A well-known endocrine condition in women is polycystic ovarian syndrome. Objective: To evaluate the effects of Vitamin D in one hundred eighty girls with PCOS. Methods: The investigation is a randomized control trial that is double-blinded. The study took place at the Ali Medical Hospital in Islamabad. The goal of the study was still to determine how adding vitamin D affected the metabolic and endocrine parameter plasma glucose in females with polycystic ovarian syndrome. This experiment had a 24-week base period and a 12-week follow-up period. Result: In this trial, no important difference of Vitamin-D addition on main variable AUCgluc and additional secondary variables metabolic and endocrine parameters were observed. There was only exclusion of a substantial reduction in plasma glucose during OGTT after 1 hr. Moreover, no improvement in PCOS or menstrual frequency in experimental group was observed. Conclusions: The study found no substantial impact of Vitamin-D supplementation on plasma glucose and on metabolic or endocrine parameters but during OGTT plasma glucose was found to be reduced after 1 hour.


Sirmans SM and Pate KA. Epidemiology, diagnosis, and management of polycystic ovary syndrome. Clinical Epidemiology. 2013 Dec; 6:1-13. doi: 10.2147/CLEP.S37559

Teede H, Deeks A, Moran L. Polycystic ovary syndrome: a complex condition with psychological, reproductive and metabolic manifestations that impacts on health across the lifespan. BMC Medicine. 2010 Jun; 8:41. doi: 10.1186/1741-7015-8-41

Wehr E, Trummer O, Giuliani A, Gruber HJ, Pieber TR, Obermayer-Pietsch B. Vitamin D-associated polymorphisms are related to insulin resistance and vitamin D deficiency in polycystic ovary syndrome. European Journal of Endocrinology. 2011 May; 164(5):741. doi: https://doi.org/10.1530/eje-11-0134

He C, Lin Z, Robb SW, Ezeamama AE. Serum Vitamin D Levels and Polycystic Ovary syndrome: A Systematic Review and Meta-Analysis. Nutrients. 2015 Jun; 7(6):4555-77. doi: 10.3390/nu7064555

Thomson RL, Spedding S, Buckley JD. Vitamin D in the aetiology and management of polycystic ovary syndrome. Clinical Endocrinology. 2012 Sep; 77(3):343-50. doi: 10.1111/j.1365-2265.2012.04434.x

Belenchia AM, Tosh AK, Hillman LS, Peterson CA. Correcting vitamin D insufficiency improves insulin sensitivity in obese adolescents: a randomized controlled trial. The American Journal of Clinical Nutrition. 2013 Apr; 97(4):774-81. doi: 10.3945/ajcn.112.050013

Tzotzas T, Papadopoulou FG, Tziomalos K, Karras S, Gastaris K, Perros P, et al. Rising serum 25-hydroxy-vitamin D levels after weight loss in obese women correlate with improvement in insulin resistance. The Journal of Clinical Endocrinology and Metabolism. 2010 Sep; 95(9):4251-7. doi: 10.1210/jc.2010-0757

Selimoglu H, Duran C, Kiyici S, Ersoy C, Guclu M, Ozkaya G, et al. The effect of vitamin D replacement therapy on insulin resistance and androgen levels in women with polycystic ovary syndrome. Journal of Endocrinological Investment. 2010 Apr; 33(4):234-8. doi: 10.1007/BF03345785

Teegarden D and Donkin SS. Vitamin D: emerging new roles in insulin sensitivity. Nutrition Research Reviews. 2009 Jun; 22(1):82-92. doi: 10.1017/S0954422409389301

Ruige JB, Assendelft WJ, Dekker JM, Kostense PJ, Heine RJ, Bouter LM. Insulin and risk of cardiovascular disease: a meta-analysis. Circulation. 1998 Mar; 97(10):996-1001. doi: 10.1161/01.cir.97.10.996

Lillioja S, Mott DM, Spraul M, Ferraro R, Foley JE, Ravussin E, et al. Insulin resistance and insulin secretory dysfunction as precursors of non-insulin-dependent diabetes mellitus. Prospective studies of Pima Indians. The New England Journal of Medicine. 1993 Dec; 329(27):1988-92. doi: 10.1056/NEJM199312303292703

Irani M and Merhi Z. Role of vitamin D in ovarian physiology and its implication in reproduction: a systematic review. Fertility and sterility. 2014 Aug; 102(2):460-8. doi: 10.1016/j.fertnstert.2014.04.046

Lerchbaum E and Obermayer-Pietsch B. Vitamin D and fertility: a systematic review. European Journal of Endocrinology. 2012 May; 166(5):765-78. doi: 10.1530/EJE-11-0984

Weisman Y, Harell A, Edelstein S, David M, Spirer Z, Golander A. 1 alpha, 25-Dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 in vitro synthesis by human decidua and placenta. Nature. 1979 Sep; 281(5729):317-9. doi: 10.1038/281317a0

Parikh G, Varadinova M, Suwandhi P, Araki T, Rosenwaks Z, Poretsky L, et al. Vitamin D regulates steroidogenesis and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells. Hormone and Metabolic Research. 2010 Sep; 42(10):754-7. doi: 10.1055/s-0030-1262837.

Legro RS, Arslanian SA, Ehrmann DA, Hoeger KM, Murad MH, Pasquali R, et al. Endocrine Society. Diagnosis and treatment of polycystic ovary syndrome: an Endocrine Society clinical practice guideline. Journal of Clinical Endocrinology and Metabolism. 2013 Dec; 98(12):4565-92. doi: 10.1210/jc.2013-2350.

Pergialiotis V, Karampetsou N, Panagopoulos P, Trakakis E, Papantoniou N. The effect of Vitamin D supplementation on hormonal and glycaemic profile of patients with PCOS: A meta-analysis of randomised trials. International Journal of Clinical Practice. 2017 Jun; 71(6). doi: 10.1111/ijcp.12957

Moher D, Hopewell S, Schulz KF, Montori V, Gøtzsche PC, Devereaux PJ, et al. CONSORT 2010 explanation and elaboration: updated guidelines for reporting parallel group randomised trials. BMJ. 2010 Mar; 340:c869. doi: 10.1136/bmj.c869.

Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, et al. Endocrine Society. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. Journal of Clinical Endocrinology and Metabolism. 2011 Jul; 96(7):1911-30. doi: 10.1210/jc.2011-0385.

Rotterdam ESHRE/ASRM-Sponsored PCOS consensus workshop group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reproduction. 2004 Jan; 19(1):41-7. doi: 10.1093/humrep/deh098

Jamilian M, Foroozanfard F, Rahmani E, Talebi M, Bahmani F, Asemi Z. Effect of two different doses of vitamin D supplementation on metabolic profiles of insulin-resistant patients with polycystic ovary syndrome. Nutrients. 2017 Nov; 9(12):1280. doi: 10.3390/nu9121280

Barrera D, Avila E, Hernández G, Halhali A, Biruete B, Larrea F, et al. Estradiol and progesterone synthesis in human placenta is stimulated by calcitriol. The Journal of Steroid Biochemistry and Molecular Biology. 2007 Mar; 103(3-5):529-32. doi: 10.1016/j.jsbmb.2006.12.097

Maktabi M, Chamani M, Asemi Z. The effects of vitamin D supplementation on metabolic status of patients with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled trial. Hormone and Metabolic Research. 2017 Jul; 49(07):493-8. doi: 10.1055/a-1736-8400

Raja-Khan N, Shah J, Stetter CM, Lott ME, Kunselman AR, Dodson WC, Legro RS. High-dose vitamin D supplementation and measures of insulin sensitivity in polycystic ovary syndrome: a randomized, controlled pilot trial. Fertility and Sterility. 2014 Jun; 101(6):1740-6. doi: 10.1016/j.fertnstert.2014.02.021



DOI: 10.54393/pbmj.v5i7.655
Published: 2022-07-31

How to Cite

Khattak , H. G. ., Ali Bhutto , M. ., Khan Bugti , M., Anjum , H. ., Fatima , M. ., Sabeen Ayesha, H. ., Ishtiaq , A., Farooq , A. ., & Latif , M. . (2022). Effectiveness of Dietary Supplementation in women with PCOS: A randomized-controlled trial: Effectiveness of Dietary Supplementation in women with PCOS. Pakistan BioMedical Journal, 5(7), 155–158. https://doi.org/10.54393/pbmj.v5i7.655



Original Article


Most read articles by the same author(s)