Impact of High-Intensity Exercise on Antioxidant System and Liver Enzymes
High-Intensity Exercise, Antioxidant System & Liver Enzymes
Keywords:High intensity exercise, Liver, Oxidative stress, FRAP Assay, ALT, ALP, AST
Liver is a vital organ of human body performing a variety of functions. In addition, maintenance of antioxidant system is also considered important. Objective: To observe the impact of high volume and high intensity exercise on blood redox state and enzymatic function of the liver. It was a comparative cross-sectional study conducted from January 2017 to Jan 2018 at Gomal University, Dera Ismail Khan Pakistan Methods: An observational study was conducted among twenty (40) voluntarily selected subjects. Measurement of both enzymatic functions of liver and blood redox state of the body were performed through liver functions tests (LFTS) and ferric reducing assay protocol (FRAP assay) by using blood samples collected from the subjects. Results: The collected data were analyzed through statistical package for social sciences (SPSS) version 25 by using different statistical tools such as Mean, Standard Deviation and T Score. Conclusions: Data analysis disclosed that high intensity exercise significantly affected the levels of ALT (t38= -4.369, p <0.05), ALP (t38= -.757, p >.05), AST (t38= -2.246, p < .05) and FRAP (t38=4.308, p<0.05).
M.L. Urso and P.M. Clarkson, "Oxidative stress, exercise and antioxidant supplementation",
Toxicology, vol. 189, no. 1-2, pp. 41-54, 2003.
M.C Gomez-Cabrera, E. Domenech, J.Viña, "Moderate exercise is an antioxidant:
Up-regulation of antioxidant genes by training", Free Radical Biology & Medicine, vol.
, no. 2, pp.126-131, 2008.
H.K. Vincent, J.W. Morgan, K.R. Vincent, "Obesity exacerbates oxidative stress levels after
acute exercise. Medical Science Sports Exercise, vol. 36, pp. 772-779, 2004.
A.J. Wadley, J.J.V. Van Zanten, S. Aldred, "The interactions of oxidative stress and
inflammation with vascular dysfunction in ageing: the vascular health triad", Age, vol. 35,
no. 3, pp. 705-718, 2013.
J. Martinović, V. Dopsaj, S.J. Kotur et al., "The physiological significance of oxidative
stress in elite volleyball players" In V. Koprivica and I. Juhas (Eds.), International
scientific conference Competitions and Athletes' Preparation). Belgrade, Serbia: Fakultet
sporta; pp. 365−369. 2009.
J. Hollander, R. Fiebig, M. Gore et al. "Superoxide dismutase gene expression is activated by
a single bout of exercise in rat skeletal muscle ", Europian Journal of Physiology, vol. 442
no.3, pp. 426-434, 2004.
Berzosa, I. Cebrian, L. Fuentes-Broto L, et al., Acute exercise increases plasma total
antioxidant status and antioxidant enzyme activities in untrained men, Journal of
Biomedicine & Biotechnology, pp. 1-7, 2011.
C.E. Cooper, N.B. Vollaard, T. Choueiri, M.T. Wilson," Exercise, free radicals and oxidative Stress" Biochemical Society Transaction, vol. 30, pp. 280−285, 2002.
C. Leeuwenburgh and J.W. Heinecke, "Oxidative stress and antioxidants in exercise, Current
Medicinal Chemistry, vol. 8, no. 7, 829-838, 2001.
R. J. Bloomer, A.H. Goldfarb, Wideman L, et al., "Effects of acute aerobic and anaerobic
exercise on blood markers of oxidative stress. Journal of Strength Condition Research,
vol.19, no. 2, pp. 276-285, 2005.
J. Finaud, G. Lac, E. Filaire, "Oxidative stress, Sports medicine, vol. 36, no. 4, 327-358,
S. Li, T. Hor-Yue Tan, N. Wang, et al., "The Role of Oxidative Stress and Antioxidants in
Liver Diseases" International Journal Molecular Sciences, vol. 16, 26087-26124, 2015.
A. Dayan, A. Rotstein, I. Pinchuk, et al., "Effect of a short-term graded exhaustive exercise
on the susceptibility of serum lipids to oxidation" International Journal of Sports Medicine, vol. 26, pp. 732-738, 2005.
A. M. Clark, L. Hartling, B. Vandermeer, F.A. McAlister, "Meta-analysis: secondary
prevention programs for patients with coronary artery disease. Annals of International Medicine, vol. 143, pp. 659-72, 2005.
G. Schumann, R. Bonora, F. Ceriotti, et al, "IFCC primary reference procedures for the
measurement of catalytic activity concentrations of enzymes at 37 °C: Part 4. Reference
procedure for the measurement of catalytic concentration of alanine-aminotransferase".
Clinical Chemistry Laboratory Medicine, vol. 40, pp. 718-733, 2002.
G, Schumann, R, Kauke, F. Canaias, et al, "IFCC primary reference procedures for the
measurement of catalytic activity concentrations of enzymes at 37 °C. Part 9: Reference
procedure for the measurement of catalytic concentration of alkaline phosphatase".
Chemical Laboratory Medicine, no. 49, pp.1439-46, 2011.
I.F. Benzie and J.J. Strain, "The ferric reducing ability of plasma (FRAP) as a measure of "
antioxidant power the FRAP assay". Analytical Biochemistry, vol. 239, no. 1, pp. 70-76,
J. Pettersson, U. Hindorf , P. Persson, et al, "Muscular exercise can cause highly
pathological liver function tests in healthy men". Brazilian Journal of Clinical
Pharmacology, vol. 65, no. 2, pp. 253-259, 2008.
M.H. Sjogren."Transaminase Levels and Vigorous Exercise", Gastro Hepatology, vol. 3,
no. 12, pp. 913-914, 2007.
M.A. Bakowski, V, Braun, J.H. Brumell , "Salmonella‐containing vacuoles: directing
traffic and nesting to grow. Traffic, vol. 9, no. 12, pp. 2022-2031, 2008.
K.E. Fallon, G. Sivyer, K, Sivyer, A, Dare, "The Biochemistry of runners in a 1600km
ultra marathon". Brazilian Journal of Sport Medicine, vol. 33, pp. 264-269, 1999.
V. Sharma, A. Sharma, L. Kansal. "The effect of oral administration of Allium sativum
extracts on lead nitrate induced toxicity in male mice". Food & Chemical Toxicology, vol.
, no. 3, pp. 928-936, 2010.
B.E. Statland, P, Winkel, H. Bokelund, "Factors contributing to intra-individual variation
of serum constituents: 2. Effects of exercise and diet on variation of serum constituents in
healthy subjects". Clinical Chemistry, vol. 19, no. 12, pp. 1380-1383, 1973.
X.G. Liu, Y.J. Zhou, T.Y.C. Liu, J. Q. Yuan. "Effects of low-level laser irradiation on rat skeletal muscle injury after eccentric exercise". Photo-medicine & Laser Surgery, vol. 27, no.6, pp. 863-869, 2009.
Z. Gholamnezhad, M.H. Boskabady, M. Hosseini, et al., "Evaluation of immune response after moderate and overtraining exercise in Westar rat". Iranian Journal of Basic Medical Sciences, vol. 17, no. 1, 1-7, 2014.
J. Stevanović, J. Beleza, P. Coxito, A. Ascensão, J. Magalhães. "Physical exercise and liver "fitness": Role of mitochondrial function and epigenetics-related mechanisms in non-alcoholic fatty liver disease". Molecular metabolism, vol. 32, pp. 1-14. 2020.
J. Beleza, D. Rizo-Roca, A. Ascensão, J. Magalhães. "Targeting mitochondria with sweat: improving mitochondrial function with physical activity". Mitochondrial Biology and Experimental Therapeutics, pp. 379-406, 2018.
D. Houghton, C. Thoma, K. Hallsworth, S. Cassidy, T. Hardy, A.D. Burt, M.I. Trenell. "Exercise reduces liver lipids and visceral adiposity in patients with nonalcoholic steatohepatitis in a randomized controlled trial". Clinical Gastroenterology and Hepatology, vol. 15, no.1, pp. 96-102, 2017.
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