Awareness and Preventive Practices Regarding Hospital-Acquired Infections among Medical Staff in Tertiary Care Hospitals: A Descriptive Cross-Sectional Questionnaire-Based Study
Preventive Practices Regarding Hospital-Acquired Infections among Medical Staff
DOI:
https://doi.org/10.54393/pbmj.v9i4.1388Keywords:
Cross-infection, Infection Control , Hand Hygiene, Personal Protective Equipment, Health Knowledge, Attitudes, Practice, Health PersonnelAbstract
Hospital-acquired infections (HAIs) continue to be a major public health threat, leading to significant morbidity, mortality, and health care expenditures worldwide. They have been linked to extended hospital stays and increasing antimicrobial resistance. Doctors, nurses, and other medical personnel are IPC actors because of their regular interactions with patients and protocol implementation responsibilities. Objectives: To assess IPC awareness, adherence to preventive measures, and challenges to effective implementation. Methods: This descriptive cross-sectional survey was conducted from January to May 2025 in three private tertiary care hospitals. 220 registered medical staff were administered a validated structured questionnaire consisting of a set of questions related to demographics, knowledge items, self-reported practices, and perceived barriers. SPSS 25 software was used for data processing and descriptive statistics, and Pearson correlation was used to examine the relationship between awareness and practice scores. Results: The following criteria were applied to classify scores as 'good' (≥75%), 'moderate' (50-74%) or 'poor' (<50%). These thresholds were chosen because they are consistent with the literature and help to compare the results of different studies. Conclusions: There is a significant discrepancy between knowing and doing. We recommend strengthening IPC education, systematic competency audits, and policy adjustments to ease workload and strengthen institutional support for IPC to enhance awareness and preventive practices among Medical Staff.
References
AlOtaibi M, Alrashedi HN, Alasmari HA, Hendy A, Ibrahim RK. Optimizing Infection Control: Evaluating Nurses' Knowledge and Practices for Preventing Infections in Mechanically Ventilated Patients. Infection, Disease and Health. 2025 Aug; 30(3): 194-202. doi: 10.1016/j.idh.2025.02.005. DOI: https://doi.org/10.1016/j.idh.2025.02.005
Althiyabi FS, Khuded FM, Alzaidi FM, Alswat AS, Alotaibi FS, Alotaibi WS et al. Assessment of Nursing Knowledge and Practice Toward Prevention of Acquired Infections in the Emergency Department of King Faisal Medical Complex in Taif. Sage Open Medicine. 2024 Jan; 12: 20503121231222341. doi: 10.1177/20503121231222341. DOI: https://doi.org/10.1177/20503121231222341
Harun MG, Sumon SA, Rahman A, Anwar MM, Islam MS. Infection Prevention and Control Perspective and Practices Among Healthcare Workers in Bangladesh: A Multicenter Cross Section. Antimicrobial Stewardship and Healthcare Epidemiology. 2023 Jun; 3(2): 74-5. doi: 10.1017/ash.2023.326. DOI: https://doi.org/10.1017/ash.2023.326
Alshagrawi S and Alhodaithy N. Risk Factors of Healthcare-Associated Infection Among Healthcare Workers in Intensive Care Units: A Multicenter Cross-Sectional Study. Public Library of Science One. 2024 Dec; 19(12): e0314796. doi: 10.1371/journal.pone.0314796. DOI: https://doi.org/10.1371/journal.pone.0314796
Di Gennaro F, Segala FV, Papagni R, De Vita E, Guido G, Frallonardo L et al. Knowledge, Practices, Educational Needs and Hospital Engagement in Infection Prevention and Control Among Italian Healthcare Workers and Students: Results from a National Multicenter Survey. JAC-Antimicrobial Resistance. 2025 Jun; 7(3): 081. doi: 10.1093/jacamr/dlaf081. DOI: https://doi.org/10.1093/jacamr/dlaf081
Ayed A, Malak MZ, Ayed M, Allayed R, Shouli M. Knowledge, Attitudes, and Practices Toward Infection Control Precautions Among Nurses in Palestinian Hospitals. International Journal of Nursing Education Scholarship. 2024 Jan; 21(1): 20230117. doi: 10.1515/ijnes-2023-0117. DOI: https://doi.org/10.1515/ijnes-2023-0117
Behzadmehr R, Doostkami M, Sarchahi Z, Dinparast Saleh L, Behzadmehr R. Radiation Protection Among Health Care Workers: Knowledge, Attitude, Practice, and Clinical Recommendations: A Systematic Review. Reviews on Environmental Health. 2021 Jun; 36(2): 223-34. doi: 10.1515/reveh-2020-0063. DOI: https://doi.org/10.1515/reveh-2020-0063
Albarrak AI, Mohammed R, Al Elayan A, Al Fawaz F, Al Masry M, Al Shammari M et al. Middle East Respiratory Syndrome: Comparing the Knowledge, Attitude and Practices of Different Health Care Workers. Journal of Infection and Public Health. 2021 Jan; 14(1): 89-96. doi: 10.1016/j.jiph.2019.06.029. DOI: https://doi.org/10.1016/j.jiph.2019.06.029
Foga Sebro S, Birhanu M, Bilal A, Sahle T. Knowledge and Practice Toward Hospital-Acquired Infections Prevention and Associated Factors Among Nurses Working at University Referral Hospitals in Southern Nations, Nationalities, and Peoples’ Region, Ethiopia 2021. SAGE Open Medicine. 2023 Jan; 11: 20503121221149362. doi: 10.1177/20503121221149362. DOI: https://doi.org/10.1177/20503121221149362
Khan D, Waqar F, Azim N, Khan O, Sohail A, Azim SN et al. Reduction of Hospital-Acquired Infections Through a Nursing Education Program: A Quality Improvement Project on the Sensitization of Nursing Staff Toward Infection Control in Neonates. Cureus. 2024 Jun; 16(6): 1-9. doi: 10.7759/cureus.62656. DOI: https://doi.org/10.7759/cureus.62656
Hong Q and Xu Y. Evaluating Hand Hygiene Knowledge, Attitudes, and Practices Among Healthcare Workers in Post-Pandemic H1N1 Influenza Control: A Cross-Sectional Study from China. Frontiers in Public Health. 2024 Sep; 12: 1432445. doi: 10.3389/fpubh.2024.1432445. DOI: https://doi.org/10.3389/fpubh.2024.1432445
Daba C, Atamo A, Gebretsadik Weldehanna D, Oli A, Debela SA, Luke AO et al. Infection Prevention and Control Compliance of Healthcare Workers Towards COVID-19 in Conflict-Affected Public Hospitals of Ethiopia. BioMed Journal Open. 2023 Dec; 13(12): e074492. doi: 10.1136/bmjopen-2023-074492. DOI: https://doi.org/10.1136/bmjopen-2023-074492
Campo LK and Remon AR. Knowledge and Practices of Nurses on the Prevention and Control of Healthcare-Acquired Infections in a Private Tertiary Hospital in Baguio City. Acta Medica Philippina. 2025 Feb; 59(3): 92.
Batran R, Ayed A, Batran A, Ejheisheh MA, Alassoud B, Hayek MF et al. Determinants of Nurses’ Compliance with Infection Prevention and Control Practices in Critical Care Units. SAGE Open Nursing. 2025 Apr; 11: 23779608251339193. doi: 10.1177/23779608251339193. DOI: https://doi.org/10.1177/23779608251339193
Duwal S, Budhathoki L, Dhaubanjar M, Rijal D, Acharya P. Hand Hygiene Practice Compliance Among Healthcare Workers in a Tertiary Healthcare Hospital in Kathmandu, Nepal. Public Library of Science Global Public Health. 2024 Aug; 4(8): e0003322. doi: 10.1371/journal.pgph.0003322. DOI: https://doi.org/10.1371/journal.pgph.0003322
Liaqat R, Satara A, Bibi S, Shameem M, Perveen N. Assessment of Knowledge and Practices of Nosocomial Infection Among Nurses of Jinnah Hospital Lahore, Punjab-Pakistan: Nosocomial Infection Among Nurses. Nursearcher. 2025 Jun; 30: 42-7. doi: 10.54393/nrs.v5i2.150. DOI: https://doi.org/10.54393/nrs.v5i2.150
Kanwal A, Latif A, Bibi A, Manzoor N, Kousar F, Maqbool B. Knowledge and Practice of Nurses Regarding Infection Control Measures in Tertiary Care Hospital. Biological and Clinical Sciences Research Journal Publishers. 2025; 6(1): 134-7. doi: 10.54112/bcsrj.v6i1.1536. DOI: https://doi.org/10.54112/bcsrj.v6i1.1536
Datta S, Kumar A, Chatterjee B. Hand Hygiene Compliance Among Healthcare Workers at A Tertiary Care Hospital in Eastern India. Journal of Preventive and Complementary Medicine. 2025 Jul; 4(2): 76-83.
Kamara GN, Sevalie S, Molleh B, Koroma Z, Kallon C, Maruta A et al. Hand Hygiene Compliance at Two Tertiary Hospitals in Freetown, Sierra Leone, In 2021: A Cross-Sectional Study. International Journal of Environmental Research and Public Health. 2022 Mar; 19(5): 2978. doi: 10.3390/ijerph19052978. DOI: https://doi.org/10.3390/ijerph19052978
Cheng BT, Ali RA, Collet JC, Towell TD, Han G, Keen D et al. Barriers to Healthcare-Worker Adherence to Infection Prevention and Control Practices in British Columbia During the Coronavirus Disease 2019 (COVID-19) Pandemic: A Cross-Sectional Study. Infection Control and Hospital Epidemiology. 2024 Apr; 45(4): 474-82. doi: 10.1017/ice.2023.242. DOI: https://doi.org/10.1017/ice.2023.242
Olatade MJ and Ifeoluwa A. Knowledge and Preventive Practices of Nosocomial Infections Among Health Workers in Two Selected Tertiary Hospitals in Ogun State. International Journal of Caring Sciences. 2021 Jan; 14(1): 174.
Khatrawi EM, Prajjwal P, Farhan M, Inban P, Gurha S, Al‐ezzi SM et al. Evaluating the Knowledge, Attitudes, and Practices of Healthcare Workers Regarding High‐Risk Nosocomial Infections: A Global Cross‐Sectional Study. Health Science Reports. 2023 Sep; 6(9): e1559. doi: 10.1002/hsr2.1559. DOI: https://doi.org/10.1002/hsr2.1559
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