Methicillin-Resistant Staphylococcus Aureus (MRSA) Epidemiology and Antibiotics Susceptibility Profile Isolated from Different Clinical Samples in Tertiary Care Hospital

MRSA Epidemiology and Antibiotics Susceptibility Profile


  • Wajid Ali Department of Pharmacology, Ayub Medical College Abbottabad
  • Shah Zaman Department of Pharmacology, Peshawar Medical College
  • Zakia Subhan Department of Pharmacology, KMU-IMS Kohat
  • Abdur Razaq Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical University Peshawar
  • Muhammad Nabi Institute of Pharmaceutical Sciences Khyber Medical university (IPS.KMU)
  • Maria Khattak Department of Pharmacology, Khyber Medical University
  • Nabiha Naeem Department of Life Sciences, School of Science, University of Management and Technology (UMT), Lahore, Pakistan
  • Dua-E-Jamila Khurrum Department of Life Sciences, School of Science, University of Management and Technology (UMT), Lahore, Pakistan
  • Sudhair Abbas Bangash Faculty of Life Science, Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, Pakistan
  • Irfan Ullah Department of Life Sciences, School of Science, University of Management and Technology (UMT), Lahore, Pakistan



Methicillin-Resistant, Staphylococcus aureus, Antibiotics, Susceptibility, Tertiary Care


Staphylococcus aureus is a versatile bacterium that causes a wide range of diseases in humans and animals. Objective: To determine the occurrence and antibiotic sensitivity profiles of Methicillin-Resistant Staphylococcus aureus (MRSA) isolates directly from the clinical samples. Methods: Individuals from various subgroups of the District Peshawar provided three different clinical specimens that are pus, body fluids and blood. Plasma, Macconkey and Cysteine Lactose Electrolyte Deficient agar (CLED) agar were used to prepare each specimen in the usual method. Gram staining test, catalase, and coagulase were used to identify and confirm S. aureus. The conventional "Kirby-Bauer disc" diffusion method was used to confirm MRSA antibiotic resistance patterns to several antibiotics. Results: A maximum of 750 diagnostic samples were evaluated and 50 (6.37%) were found to be positive for MRSA, with 33 (72%) coming from pus samples, 9 (19%) from fluid samples, and 6 (12%) from blood samples. Males had a higher prevalence of MRSA strains (69%) than females (31%). Most MRSA strains were completely resistant to different type of antibiotics e.g. penicillin, oxacillin, and ampicillin, while remaining completely susceptible to linezolid, teicoplanin, & vancomycin. Other anti-microbials to which MRSA strains were resistant are ceftriaxone (78.88%), cefoxitin (65.55%), erythromycin (83.33%), clindamycin (72.22%), co-amoxiclav (76.66%), fusidic acid (67.77%), and gentamycin (83.33%) (74.4%). Conclusions: This study found that the frequency of MRSA in Pakhtunkhwa is lower in comparison to that reported in other regions of Pakistan. Moreover, because MRSA is multi-drug-resistant, culture sensitivity testing should be conducted to determine the best antibiotic to use to treat MRSA infection


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DOI: 10.54393/pbmj.v5i5.455
Published: 2022-05-31

How to Cite

Ali, W. ., Zaman, S. ., Subhan, Z. ., Razaq, A., Nabi, M. ., Khattak, M. ., Naeem, N. ., Khurrum, D.-E.-J. ., Abbas Bangash, S. ., & Ullah, I. . (2022). Methicillin-Resistant Staphylococcus Aureus (MRSA) Epidemiology and Antibiotics Susceptibility Profile Isolated from Different Clinical Samples in Tertiary Care Hospital: MRSA Epidemiology and Antibiotics Susceptibility Profile. Pakistan BioMedical Journal, 5(5), 108–112.



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